Earth Science Institute of Orleans, France UMR 6531 of CNRS - University of Orléans Group « Organic Matter in water, sols, sediments and rocks » Personal involved in RECIPE : Fatima Laggoun Laure Comont (PhD student) Jean-Robert Disnar Patrick Albéric (DOC, water chemistry) Christian Défarge (µmorphology - cryo-SEM) Nathalie Lottier (GC-MS / Capillary eletrophoresis) Marielle Hatton (C, N, S & palynofacies preparation) Charquemont, Recipe meeting 23-27th Oct 2003

Appointment of staff and students to RECIPE & relevant problems :  Application for many calls: (i) PhD grants from public Dpts, (ii) Marie Curie post-doc grant (6th Framework Prog.)  Oct to Nov. 2002: a PhD student A. Fleury (2 Jura sites)  Oct. 1 st 2003: Laure Comont [3 years PhD grant from Research Dpt of Local Adminitr. District (Region Centre)]  Eval. MC grant: C. Applicant: Martin Kainz, Univ Victoria, Canada. Project: Biomolecular source identification of OM in peatland ecosystems (lipid biomarkers, i.e. fatty acid methyl esters)  Philipp Steinmann: invited staff at ISTO in the framework of RECIPE (1 month (April or May 2004) fund from Univ Orleans). Project: (i) porewater sampling by peepers & isotopic analyses of CO 2, CH 4 and acetates; (ii) Archaea biomarkers. Charquemont, Recipe meeting 23-27th Oct 2003

Analysis progress:  Sampling on Oct sites in the Jura: Le Russey (France): 5 cores (70 Samples) La Chaux d’Abel (Switzerland): 6 cores (91 Samples)  Analyses: CNS analyses, identification & counting of organic fragments, counting of bacteria, sugar analyses of peat samples and vegetation (8 sphagnum sp., 1 polytrichum, 2 Erioph. sp.).  Participation to congresses: 9 th Congress of Sedimentology (Oct., 14-16th 2003, Bordeaux) 7 th Meeting of Soil Study (Oct., 22-24th 2002, Orleans)

Sampling in la Chaux d’Abel

C/N ratios and microscopic counting Chaux d’Abel/fen (minetrophic zone)-Newly restaured CA.1 Sphagnum & Polytrichum CA.2 Sphagnum & Eriophorum

C/N ratios and microscopic counting CA.5 Sphagnum & PolytrichumCA.4 Sphagnum & Eriophorum Chaux d’Abel/bog (ombrotrophic zone) Newly restaured Charquemont, Recipe meeting 23-27th Oct 2003

C/N ratios and microscopic counting Chaux d’Abel Intact zone ( CA 6) Charquemont, Recipe meeting 23-27th Oct 2003

Regeneration ? C/N ratios and microscopic counting (Le Russey) Ry.B – no regeneration ? Ry.D – more advanced stage

C/N ratios and microscopic counting (Le Russey) Ry.I – Intact peat Regeneration Charquemont, Recipe meeting 23-27th Oct 2003

S. rubellum Rhm Gal % Biomarkers of organic sources (sugar analyses) ERIOPHORUM XyloseArabinose POLYTRICHUM Mannose H SPHAIGNES RhamnoseGalactose ERIOPHORUM Ara Xyl % POLYTRICHUM Man H % S. fallax Rhm Gal % vegetation Charquemont, Recipe meeting 23-27th Oct 2003

sugar analyses ERIOPHORUM POLYTRICHUM SPHAIGNES XyloseArabinose Mannose H RhamnoseGalactose La Chaux d’Abel / bog - exploited zone % Arabinose et Xylose 2 Profondeur (cm) % Mannose 3 Profondeur (cm) % Rhamnose et Galactose 1 Profondeur (cm) Charquemont, Recipe meeting 23-27th Oct 2003

ERIOPHORUM POLYTRICHUM SPHAIGNES XyloseArabinose Mannose H RhamnoseGalactose La Chaux d’Abel / INTACT ZONE % Glucose Profondeur (cm) 1 % Xylose Profondeur (cm) 1 % Man, Ara, Fuc et Rib Profondeur (cm) sugar analyses Charquemont, Recipe meeting 23-27th Oct 2003

bacteria counting Observations x1000 en épifluorescence 50  m 5  m 3  m Charquemont, Recipe meeting 23-27th Oct 2003

bacteria counting (La chaux d’Abel) Taille échantillon Taille échantillon de départ de départ Concentration en bactéries/g de bactéries/g de tourbe fraîche BIOMASSECarbone Biovolume INTACT Zone Profondeur (cm) Abondance b/g.ech.sec.Biomasse mgC.g Profondeur (cm) EXPLOITED Zone Abondance b/g.ech.sec.Biomasse mgC.g CA 5 CA 6 Charquemont, Recipe meeting 23-27th Oct 2003

Bacteria counting (Le Russey) Ry.B – Exploited zone Charquemont, Recipe meeting 23-27th Oct 2003

Ry.D – Exploited zone Bacteria counting (Le Russey) Transition (C/N, %OF) Regeneration Charquemont, Recipe meeting 23-27th Oct 2003

Ry.I – Intact zone Bacteria counting (Le Russey) Regeneration Charquemont, Recipe meeting 23-27th Oct 2003

Replicates ? (Ry A & B: less advanced regeneration stage) Charquemont, Recipe meeting 23-27th Oct 2003