Avantages de l'utilisation du Re-Hydra Pro en alimentation animal

Présentation au sujet: "Avantages de l'utilisation du Re-Hydra Pro en alimentation animal"— Transcription de la présentation:

1 Avantages de l'utilisation du Re-Hydra Pro en alimentation animal

2 Qu’est-ce c’est rehidratation Pellet PLUS+?
Pellet Plus+ permet de récupérer les pertes dans l’aliment a partir de l’aplication d’humidité atomisée au mélangeur et en augmentant l’eficacité de la machine à pellet. Possible application à partir de senseurs: Les senseurs pour déterminer l’humidité sont placés sur tout le procès productif pour mesurer l’humidité résiduelle dans un aliment pelletisé. Application corrective de l’agent humidifiant Re-Hydra Pro® pour arriver aux valeurs fixés. L’application change en fonction de l’humidité captée par les senseurs.

3 Senseur au réfroidisseur (optional)
Plan d’application après le monitorage de l’humidité avec le système Pellet PLUS+ d’ADIVETER Bactéricide Antifongique Rehidratation + antifongique Senseur au réfroidisseur (optional)

4 Eau avec Re-Hydra Prol®
Re-Hydra Pro® c’est un agent humidificateur avec proprietés antifongiques. Cet additive réhydratant ameliore le rendement de la machine à pellet et la qualité du pellet. La fonction antifongique Spécifications du produit Dosage g par tonne de matière prèmière ou aliment selon l’objectif antifongique. Nom chemique Acide propionique max. 60% Ammonium propionate, max. 27% Mono and diacylglycerides of food fatty acids max. 10% Inert carrier, up to 100% Physico-chemical Properties Color: Blue Appearance: Clear Odor: Irritating Presentation: liquid (IBC 1000 l) Apparent density: 1.06 kg/ l p.H: Dissolves in water with no limit Re-Hydra Pro® est complémenté avec agents tensioactives pour assurer la meilleure dispersion des ingrédients actifs et l’homogénéité aux matières premières ou au mélange. Amélioration de l’eau absorbé dans le mélange – poids finale plus haute Eau pas disponible pour les mycoses Proportion de …plus basse et meilleure durabilité du pellet. Control des mycoses Amelioration des rendements Eau avec Re-Hydra Prol®

5 Avantages pratiques de l’utilisation du Re-Hydra Pro®

6 Résumé des avantages du programme de Réhydratation
PHYSIQUES Humidité finale plus haute (+ poids) sans augmenter l’Aw Rétention plus haute d’eau, fixé aux pellets Prés du 82% de l’eau ajouté reste à l’aliment: Poids final plus haute et stable après le stockage.

7 T0 + 0.82% + 1.64% T1 T2 % Pellet quality parameters
% Remaining moisture % T0 T1 T2 + 0.82% + 1.64% 11,74 12 11,5 10,92 11 10,42 10,3 10,1 10,5 Re-Hydra Pro®, au dosage de1 et 2 % de solution hydratante, augmente d’une manière proportionnelle le niveau d’humidité du pellet. Cet humidité retenue donne une diminution des pertes au niveau de l’aliment. 10 9,5 9 T1 – Control T2 - 1% water T3 - 2% water T4 - 1% HS + 2,5% RHP T5 - 2% HS + 2,5% RHP

8 Résumé des avantages du programme de Réhydratation
PRODUCTIVE – Aliment composé Plus haute rendement (presque 10%) Consommation énergétique plus basse On peut arriver à températures plus hautes Diminution de la production de poudre

9   Génération d’un film aqueuse très fin.
Avantages du programme de Réhydratation. PRODUCTION Génération d’un film aqueuse très fin. Amélioration de la lubrification des machines. Plus quantité d’aliment accepté. Pelleting machine  Consommation énergétique plus basse (kWh/Tm) presque le 9 – 10%, par chaque 1% de solution hydratante ajouté.  Rendement plus haute (Tm/h)- près du 10%, par chaque 1% de solution hydratante ajouté.

10 T0 + 8.9% + 13.27% T1 T2 Paramètres de pelletisation t/ h
29.01 29,01 % T1 30 27,89 27.89 T2 28 25,61 25.61 26 Pour chaque 1% de solution hydratante ajoutée il y a une diminution du 10% sur le temps de production des pellets sans augmentation de la consommation d’énergie On arrive à un plateau après inclusions supérieurs au 1.5%. 24 22 20 T0 T0 - - Control Control T1 T1 - - 1% HS + 2,5% RHP T2 T2 - - 2% HS + 2,5% RHP

11 T0 - 76.2% - 150.2% T1 T2 Paramètres de pelletisation
% de pertes sur l’aliment. (Weight raw materials minus total final feed in truck) T0 % - 76.2% 0.35 0.5 % T1 T2 L’eau ajoutée permet de récupérer les pertes produites dans l’aliment pendant le broyage et la pelletisation. La rétention d’eau est du 75 au 80% environ. -0.5 -0.5 -1 -1.16 T0 T0 - - Control Control T1 T1 - - 1% HS + 2,5% RHP T2 T2 - - 2% HS + 2,5% RHP

12 Presque 8 kg d’aliment de plus
Numéros pratiques 1 tonne d’aliment: Investissement: 10 l d’eau 250 g Re-Hydra Pro® Bénéfice: Presque 8 kg d’aliment de plus Volaille €/ kg 1.17 € bénéfice Porc €/ kg 1 € bénéfice Engraissement Boeuf €/ kg 0.8 € bénéfice

13 Résumé des avantages du programme de Réhydratation
QUALITÉ du pellet Taux de gélatinisation de l’amidon plus haute. PDI-Durabilité plus haute (moins pertes). Humidité plus haute-plus de palatabilité. Production de poudre plus basse.

14 Avantages sur la qualité du pellet avec Re-Hydra Pro®
Amélioration du taux de gélatinisation de l’amidon Du 4 au 7% de plus; >20% et prêt du 30% Meilleur PDI (Taux de Durabilité du Pellet) – presque le 3% de plus: Plus haute fixation du pellet Taux de poudre plus basse Meilleur assimilation de l’énergie métabolisable à cause de la destruction de la couverture fibreuse des particules d’amidon/accès au CP/accès aux dépôts lipidiques.

15 Avantages sur la qualité du pellet avec Re-Hydra Pro®
Taux de gélatinisation de l’amidon de l’aliment Pourcentage de poudre retourné (aliment) % % 26.6 BD + 2% H20 Re-Hydra Pro® 30 30 23.17 25 25 19.02 BD + 2% H2O 20 BD + 2% H2O 15.1 20 12.01 BD + 2% H20 Re-Hydra Pro® 11.52 15 15 10 10 5 5 Diète basle Diète basale

16 Paramètres de qualité du Pellet
% Durabilité 95.2 % L’humidité effective ajoutée donne une amélioration de la durabilité du pellet (pour aliments sans addition d’autres additives à base de lignine) La valeur de la durabilité est en relation, d’une manière proportionnelle, à l’eau ajoutée. Augmente en relation au dosage effectivement appliqué. 95 93.75 93.37 94 93 92 91 90 T0 T0 - - Control Control T1 T1 - - 1% HS + 2,5% RHP T2 T2 - - 2% HS + 2,5% RHP

17 Avantages sur la qualité du pellet avec Re-Hydra Pro®
QUALITÉ Microbiologique Control microbiologique de l’aliment. (microdispersion) Diminution de la croissance fungique/bactérienne par l’ajoute de produits. Traitement antifongique de choc et protection à long-terme. (Effet combiné des acides organiques purs et des sels d’ammonium) Pas de production de Mycotoxines.

18 Propriétés antifongiques du Re-Hydra Pro®
Effet de choc (acides organiques purs per se) acide propionique (max 60%) Sels d’ammonium avec un effet à Long-terme et effet corrosif basse (pH 7.28, produit finale) Propionnate d’ammonium (max 27%) Haute microdispersion dans l’aliment des ingrédients actifs par l’utilisation du tensioactive. mono et diglycérides d’acides gras (max 10%)

19 Décompte Microbiologique ADIVETER. Jours 0, 7 et 14
Enterobacteriaceae Milliers 28,26 30 25 20 12,5075 15 8,7575 T0 - Control 10 5,0075 T1 - 0,25% H2O 0,8575 T2 - 0,5% H2O 0,2125 0,5075 5 0,01 0,0175 T3 - 0,25% H2O + RHP 0,01 0,02 0,0125 0,0125 0,0275 0,01 T4 - 0,5% H2O + RHP 7 14 jours

20 Décompte Microbiologique ADIVETER. Jours 0, 7 et 14
Fungi Milliers 13,8 14 12 10 8 3,05 T0 - Control 6 2,075 T1 - 0,25% H2O 0,1 4 1,125 0,1 T2 - 0,5% H2O 0,1 2,075 2 1,225 T3 - 0,25% H2O + RHP 0,125 0,175 0,175 T4 - 0,5% H2O + RHP 0,1 0,1 0,1 7 14 jours

21 Résumé des avantages du programme de réhydratation
PRODUCTIVE - animal Pertes plus basses Amélioration de la palatabilité Diminution de la pression microbiologique Meilleur rendement (Taux de conversion)

22 Meilleur utilisation de l’EM/CP/graisse
Advantages in ANIMAL by Re-Hydra Pro® Meilleur utilisation de l’EM/CP/graisse Control microbiologique total du cycle productif. Pertes d’énergie par fungi plus basses Production plus basse de poudre Incidence de micotoxicosis plus basse Durabilité plus haute Incidence de coliforms / Salmonella / Clostridium plus basse Diminution des pertes de l’aliment Niveaux de biosécurité plus hautes Palatabilité du pellet plus haute Amélioration du taux de conversion

23 Plus palatable CMQ plus haute+ GMQ plus haute
Juin 2004 UTILISATION DU Re-Hydra Pro® EN ALIMENT D’INICIATION POUR PORCELETS IT: UP-0106-BR CMQ, g/ d Control 200 ppm Re-Hydra Pro® 640 640 625 625 300 ppm Re-Hydra Pro® 6 6 11 11 400 ppm Re-Hydra Pro® 650 650 600 ppm Re-Hydra Pro® 579 579 600 600 525 525 476 476 GMQ, g/ d 224 240 407 423 21 to 35 d 35 to 54 d 21 to 54 d 50 100 150 200 250 300 350 400 450 220 219 207 316 354 397 273 298 3 24 344 334 550 550 469 469 459 459 500 500 446 446 450 450 405 405 400 400 350 350 263 263 249 249 300 300 234 234 240 240 230 230 250 250 200 200 150 150 100 100 50 50 21 to 35 d 35 to 54 d 21 to 54 d TC, g/g 1.67 1,67 1.7 1,62 1.62 1.55 1,55 1.54 1,54 1.51 1,51 1,49 1.49 Plus palatable CMQ plus haute+ GMQ plus haute 1.48 1,48 1.5 1,42 1.42 1,41 1.41 Poids finale plus haute 1.4 1,4 1.3 Pertes plus basses Amélioration du TC 1.12 1,12 1,11 1.11 1.06 1,06 1.1 1,1 1,1 1.1 1.1 0.9 21 to 35 d 35 to 54 d 21 to 54 d

24 Diminution jusqu’à 0,1 du TC Jusqu’à 2 Kg de plus au jour 54
Résultats pratiques en porcelets TC, g/g 1,48 1,49 1,41 1,4 21 à 54 j 1,42 1.48 1.49 1.41 1.4 1.3 1.35 1.45 1.5 1.42 Poids finale(kg) 16 16.5 17 17.5 18 18.5 16.18 16.96 17.71 18.19 18.04 Coût* par kg animal (€/ kg) 0.708 0.704 0.667 0.666 0.649 0.64 0.65 0.66 0.67 0.68 0.69 0.7 0.71 21 à 54 j Diminution jusqu’à 0,1 du TC Jusqu’à 2 Kg de plus au jour 54 21 à 54 j *Aliment pour porcelets à 0.67 €/ kg Control 300 ppm Re-Hydra Pro® 400 ppm Re-Hydra Pro® 600 ppm Re-Hydra Pro® 200 ppm Re-Hydra Pro® Diminution jusqu’à 0.06 €/ kg porcelet (jour 54)

25 Résumé des propriétés de réhydratation avec Pellet PLUS+

26 Higher proportion of water fixed to feed particles
1 Better pellet production yield (production, energetic cost) 2 Better overall pellet quality 3 Lower losses of fines (feedmill / farm) 4 Higher antifungal protection 5 Better animal performances 6

27 Efet de l’utilisation du Re-Hydra Pro® on the recovering of feed losses and Quality parameters of feed pellets To check the effect that the inclusion of Re-Hydra Pro® exerts on different parameters of pellet production and quality of final product (physico-chemical characteristics)

28 Experimental design T0 - Feed without addition of water
T1 - Feed with 1% Hydrating solution + 2.5% Re-Hydra Pro® T2 - Feed with 2% Hydrating solution + 2.5% Re-Hydra Pro® Each experimental treatment was composed of four production replicates (with the exception of treatment 0, with 8 replicates) Each replicate consisted of a pelleting of 50,000 kg (10 blendings of 5,000 kg of feed for swine fattening, each) Each experimental day included a T0 and one or two of the other experimental treatments (100,000 or 150,000 kg production, according to the schedule of production of every day) The application of Re-Hydra Pro® was carried out directly in the mixer (90%) and after cooling (10% prior to the fat addition) Pelleting temperature was set at 70% and load at 85% to directly evaluate the effect of the additive on production parameters after the improvement in the pelleting yield.

29 Production parameters recorded
Pellet Production yield (Tm / h) Total time to produce the replicate with 50,000 kg Total produced feed (kg at the truck) - % feed losses Amperage Set and controlled Feed load - entrance to the pelleting machine (%) Pelleting temperature (ºC) 7 monitored determinations per experimental replicate

30 Pellet quality parameters determined
Durability (%) 5 samples of 500 g each per experimental replicate (20 samples per treatment – for treatments T1 and T2; 40 samples for T0: total 80 samples)

31 Simulator Re-Hydra Pro®
Introduce the specific conditions for each customer in the upper spaces and the rest of the data will be obtained

32 Use of Re-Hydra Pro® in starter feeds for piglets - IT: UP-010604-BR
Objectives To study the effect of the addition of a re-hydrating mixtre (at growing doses from 200 to 600 ppm) on the performances of the piglets. Main results Significant improvements in the performances were obtained with increasing doses of the use of the re-hydrating mixture.

33 21 days of age at start (avg weight: 7.1 ± 1.4 kg)
June 2004 USE OF Re-Hydra Pro® IN STARTER FEEDS FOR PIGLETS IT: UP-0106-BR Objectives To evaluate the use of Re-Hydra Pro® on the performances of piglets from 21 to 54 d of age Materials and Methods Experimental Animals 240 crossed piglets (Pietrain*Large White x Landrace*Large White), half males and half females 21 days of age at start (avg weight: 7.1 ± 1.4 kg) Experimental facilities Farm of the Dept. of Animal Production of the ETSI Agrónomos of the Universidad Politécnica de Madrid Departaments of 1 m2 (1x1m), with individual feeder (5 allocations) and drinking bowl Initial temperature at 32ºC, reducing it 1ºC per week until 27ºC are reached. Controlled ventilation according to the age. Continous light program throughout the experiment. Ingredient composition of the Experimental diets Ingredients Pre - Starter Starter Barley - 30.0 National corn - 12.4 Extruded corn 48.7 15.0 Roasted soybean 10.4 6.94 Fish meal LT 10.7 3.00 Soybean 44 4.72 21.0 Acid buttermilk 3.79 - Sweet buttermilk 17.6 2.94 Soybean oil - 3.35 L - lysine HCl (78%) 0.23 0.32 L - threonine 0.12 0.18 L - tryptophan 0.04 0.02 Methionine OH 0.19 0.22 Limestone 0.67 0.68 Bicalcium phosphate 0.16 1.22 Salt 0.10 0.32 Other 2.60 2.40 Experimental design Re-hydrating Solution of Dose of Treatment mixture Re-Hydra Pro Re-Hydra Pro (% diet) (%) (g/ Tm) Control --- --- --- A 1 2 200 B 1 3 300 C 2 2 400 D 2 3 600

34 ADI, g/ d FCR, g/g ADG, g/ d CONCLUSIONS
June 2004 USE OF Re-Hydra Pro® IN STARTER FEEDS FOR PIGLETS IT: UP-0106-BR Control 200 ppm Re-Hydra Pro® 300 ppm Re-Hydra Pro® 400 ppm Re-Hydra Pro® 600 ppm Re-Hydra Pro® ADI, g/ d FCR, g/g ADG, g/ d 476 476 459 459 469 469 446 446 1.49 1,49 500 500 405 405 1.48 1,48 1,5 1.5 450 450 450 450 344 344 400 400 324 324 334 334 400 400 298 298 350 350 273 273 350 350 1,45 1.45 1.42 1,42 1,41 1.41 300 300 300 300 1.4 1,4 250 250 250 250 1.4 1,4 200 200 200 200 150 150 150 150 100 100 100 100 1,35 1.35 50 50 50 50 1,3 1.3 21 to 54 d 21 to 54 d 21 to 54 d CONCLUSIONS The inclusion of Re-Hydra Pro® into the feed formulation significantly improveed intake, average daily gain and FCR during the period of 35 to 54 d and, as a main consequence, this improvement was also reflected in the global period recorded values. This improvement was higher with higher product proportions in the feed formulation

35 Cost per kg animal (€/ kg) Final weight (kg) FCR, g/g CONCLUSIONS
June 2004 USE OF Re-Hydra Pro® IN STARTER FEEDS FOR PIGLETS IT: UP-0106-BR Control 200 ppm Re-Hydra Pro® 300 ppm Re-Hydra Pro® Cost per kg animal (€/ kg) 400 ppm Re-Hydra Pro® 600 ppm Re-Hydra Pro® Final weight (kg) FCR, g/g 0.708 0.708 0.704 0.704 0.71 1.49 1,49 18.19 1,48 1.48 18.5 18.04 1.5 0.7 17.71 0.69 18 1.45 1.42 1,42 1.41 1,41 1,4 1.4 0.68 0.667 0.667 0.666 0.666 17.5 16.96 1.4 0.67 17 0.66 0.649 0.649 1.35 16.5 16.18 0.65 1.3 0.64 16 21 to 54 d 21 to 54 d 21 to 54 d 21 to 54 d CONCLUSIONS The inclusion of Re-Hydra Pro® into the feeds allowed to decrease the production costs (per kg meat) in all treatments, favoured by the performed dilution. This happened at all levels of inclusion (1% A and B, 2% C and D), against the Control treatment. Again, this improvement was higher with the highest product inclusions.

36 Use of Re-Hydra Pro® in feed for broiler chickens –
It: Apu-Br1-04 Objectives To study the effect of adding a rehydrating mixture (at 1% concentration) on performances of broiler chickens, at the Universitat Autònoma de Barcelona. Main results Similar results –even using a diluted diet- were obtained in treated animals when compared to the Control.

37 February, 2004 USE OF RE-HYDRA PRO® IN FEED FOR BROILER CHICKENS IT: APU-BR1-04 Aim A test was carried out in order to study the effects on performance parameters of incorporating 1% of the rehydrating mixture Re-Hydra Pro® in feed for broiler chickens. Materials and Methods The experiment was conducted in a shed belonging to the Universitat Autònoma de Barcelona (Cerdanyola, Barcelona). 180 male chickens were used; these birds were 7 days old when the test began. Feed for the experiment was prepared at the IRTA (Mas Bové, Reus) (table 1). The experiment included one period from 7 to 42 days of age. Each treatment was applied to 5 replicates, with 4 chickens per replicate. Feed and water were supplied ad libitum. The experimental feeds are broken down in table 1. Table 2 shows the details of the analysis of the feed. Table 1. Experimental feed Ingredients (%) A Control B Re-Hydra P Corn 50.9 Soybean meal - 48 29.6 Fat 5.74 Wheat middlings 4.99 Maize pulps 2.50 Alimet 0.09 Limestone 1.19 Bicalcium phosphate 1.60 Salt 0.31 Florafil 0.30 Vit min premix 0.25 Re-Hydra Pro 1.0 TOTAL 100 101 Table 2. Analysis of feed Parameter (%) A Control B Re-Hydra P Moisture 13.2 13.6 Ash 5.14 4.97 Crude protein 19.35 19.18

38 Average Daily Gain (g/d) Average Daily Intake (g/d)
February, 2004 USE OF RE-HYDRA PRO® IN FEED FOR BROILER CHICKENS IT: APU-BR1-04 Average Daily Gain (g/d) Average Daily Intake (g/d) 7 to 42 d 7 to 42 d Re-Hydra Pro Re-Hydra Pro Feed Conversion Ratio Cost (€) per kg meat 7 to 42 d 7 to 42 d Re-Hydra Pro Re-Hydra Pro CONCLUSIONS Inclusion in rations for broiler chickens from 7 to 42 days old of 1% Re-Hydra Pro® rehydrating mixture led to the same performance results as the control diet.

39 Effect of the use of Re-Hydra Pro® on the improvement of pelleting efficiency and feed quality
To check the effect that the inclusion of Re-Hydra Pro® exerts on different parameters of pellet production and quality of final product (physico-chemical characteristics of horses and japanese quails feeds)

40 Experimental design T0 - Feed without addition of water
T0.5 - Feed with 0.5% Hydrating solution + 2.5% Re-Hydra Pro® T1 - Feed with 1.5% Hydrating solution + 2.5% Re-Hydra Pro® Each experimental treatment was composed of two production replicates (with the exception of treatment 0.5, with 1 replicates), determined in two different days (for two different pelleting machines and two different feeds) Each replicate consisted of a pelleting of 9,000 kg (3 blendings of 3,000 kg of feed for japanese quails and horses feeds, each) Each experimental day included one replicate of T0 production and one (horses feed) or two (japanese quails) of the other experimental treatments. The application of Re-Hydra Pro® was carried out directly in the mixer Pelleting temperature was set around 45ºC and Amperage at 245 and 200 Å for pelleting machine 1 and 2, respectively, to directly evaluate the effect of the additive on production parameters after the improvement in the pelleting yield.

41 Production parameters recorded
Pellet Production yield (t / h) Amperage Set and controlled Feed load - entrance to the pelleting machine (%) Pelleting temperature (ºC) 6 monitored determinations per experimental replicate

42 Pellet quality parameters determined
Durability (%) / Moisture (%) 5 samples of 1000 g each per experimental replicate (10 samples per treatment - for feeds belonging to treatment T1; 10 samples for T0: total 20 samples)

43 Pelleting machine 1 - performance (japanese quail feed) -
day 15th december, 2004 Tt Hydr sol. Re-Hydra Pro identification t/h Amperage Temp added time pelleting T0 9,16 4456 245 51 9,28 4452 245 50 9,42 4455 240 46 10,10 4315 230 33 10,25 4311 230 34 10,38 4315 230 41 4384.0 236.7 42.5 T0.5 0.5 2.5 10,10 4535 240 33 11,21 4527 250 44 11,34 4526 240 45 11,45 4523 240 47 11,57 4524 240 48 12,07 4523 230 49 4526.3 240.0 44.3 % improvement 3,2 T1 1.5 2.5 15,00 4650 240 37 15,10 4641 240 40 15,20 4851 240 44 15,30 4853 240 47 15,40 4849 240 43 15,50 4849 230 45 16,00 4851 220 48 4792.0 235.7 43.4 % improvement 9.3

44 Improvement of the pelleting efficiency (t/h) in pelleting machine 1, japanese quails feed
+ 3.4% + 9.4% 4.79 4.8 4.53 4.6 4.38 The use of Re-Hydra Pro® efficiently improved the production yield of the pelleting machine without an increase in the energy consumption. It is necessary to consider that the quail’s feed has a much smaller diameter than other feeds. Anyway, Re-Hydra Pro® was able to improve the feed production efficiency even under such circumstances. 4.4 4.2 4 Control 0.5% Hydr sol % RHP 1.5% Hydr sol % RHP

45 Quality parameters pelleting machine 1 (japanese quails feed)
- 15th december 2004 Tto Hydr sol Re-Hydra Pro identification Moisture Durability added time T0 9,20 10.47 98.47 10.2 98.43 9,28 10.51 98.93 9,42 10.52 99.25 9,57 10.6 99.24 10.46 98.9 T1 1.5 2.5 15,30 12.19 99.12 15,40 11.72 98.53 15,50 11.34 98.81 15,55 11.24 98.66 16,00 11.04 98.78 11.51 98.8 % improvement 10.0 Ret. Applied moisture 70% Re-Hydra Pro® allowed to fix the added water in an stable way, thus recovering the feed losses generated during milling and pelleting. With T1, approximately 70% of the water added was recovered. It has to be considered that between the day of production of the feed (wed) and the day of analysis (mon), it took 5 days. Anyway, the water added remained in the feed during that period.

46 Pelleting machine 2 - performance (horse feed)
- day 16th december, 2004 Tto Sol. Hidr Re-Hydra Pro identification t/h Amperage Temp added time pelleting T0 8,25 5110 210 42 8,35 4868 190 44 8,45 4823 47 8,55 4738 9,05 4790 9,10 5118 48 4907.,8 193.3 45.8 T1 1.5 2.5 9,25 5832 49 9,40 5860 9,47 6147 46 9,55 5506 10,07 5485 10,19 5514 45 10,34 5694 5719.7 190.0 45.9 % improvement 16.5

47 Improvement of the pelleting efficiency (t/h) in pelleting machine 1, Horses feed
+ 16.5% 5.72 5.9 5.7 The use of Re-Hydra Pro® efficiently improved the production yield of the pelleting machine without an increase in the energy consumption. Diameter of the horses’ feed was much larger than quails’s. Here, the use of Re-Hydra Pro® could efficiently optimize even more the production yield of the pelleting machine: uses up to 1.5% of hydrating solution resulted in almost 17% better production performance during pelleting. 5.5 5.3 4.91 5.1 4.9 4.7 4.5 Control 1.5% Hydr sol % RHP

48 Quality parameters pelleting machine 2 (horses feed)
- 16th december 2004 Re-Hydra Pro® allowed to fix the added water in an stable way, thus recovering the feed losses generated during milling and pelleting. Again, with T1, approximately 70% of the water added was recovered. It has to be considered that between the day of production of the feed (thu) and the day of analysis (mon), it took 4 days. Anyway, the water added remained in the feed during that period, in an stable way Tt Hydr sol Re-Hydra Pro identification Moisture Durability added tiempo T0 8,35 10.07 96 8,40 9,93 96.51 8,45 10.2 96.86 8,55 10.24 96.45 10.1 96.5 T1 1.5 2.5 9,59 10.99 95.98 10,09 11.11 96.39 10,25 11.06 96.48 10,30 11.25 11.1 96.3 % mejora 9.8 Ret. Humedad Aplicada 66%

49 Effect of the use of Re-Hydra Pro® on the recovering of feed losses and microbiological parameters of feed To evaluate the effect that the inclusion of Re-Hydra Pro® exerts on different pellet production and quality of final product (physico-chemical and microbiological characteristics) To compare the characteristics of pure free water against Re-Hydra Pro®

50 Experimental design T0 - Feed without addition of water
T1 - Feed with 0.25 % water T2 - Feed with 0.5 % water T3 - Feed with 0.25 % hydrating Solution complemented with 10% Re-Hydra Pro® T4 - Feed with 0.25 % hydrating Solution complemented with 10% Re-Hydra Pro® The application of Re-Hydra Pro® was done directly on the mixer.

51 Feed sampling 4 samples 100 g feed per replicate (total: 20 samples) before application of hydration Analysis of basal moisture and Microbiological quality 4 samples 1 kg feed per treatment (total: 20 samples) the the pelleting cooling system Analysis of residual moisture Microbiological quality

52 Laboratory analyses Pellet quality Microbiological Quality
Residual moisture at days 0, 7 and 14 Density Microbiological Quality Fungal contamination (days 0, 7, 14) Analysis of Salmonella, Staphylococcus aureus, Escherichia coli, Clostridium perfringens, total enterobacteriaceae (days 0, 7, 14)

53 Microbiological count ADIVETER at day 0
Salmonella Enterobacterias E. coli Staphylococcus Levaduras Hongos Clostridium cfu/ g aureus filamentosos perfringens T0 Hydr sol Re-Hydra Pro Pre Absence 2200 Presence <10 78000 47000 Post 3400 <100 100 20 Average 857,5 10 12,5 T1 0,25 820 212,5 T2 0,5 200 4500 1225 T3 Absence <10 <100 <100 10 T3 0,25 10 Post Absence <10 Absence <10 <100 <100 <10 T3 0,25 10 Post Absence <10 Absence <10 <100 200 <10 Average Absence 10 Absence 10 100 125 10 T4 0,5 10 Post Absence <10 Absence <10 <100 <100 <10 T4 0,5 10 Post Absence <10 Absence <10 <100 <100 <10 T4 0,5 10 Post Absence <10 Absence <10 <100 <100 <10 T4 0,5 10 Post Absence 20 Absence <10 <100 <100 <10 Average Absence 12,5 Absence 10 100 100 10

54 Microbiological count ADIVETER at day 0
Enterobacteriaceae (cfu/ g) 857 213 10 13 Day 0 100 200 300 400 500 600 700 800 900 T0 - Control T1 - 0,25% H2O T2 - 0,5% H2O T3 - 0,25% H2O + RHP T4 - 0,5% H2O + RHP

55 Enterobacteriaceae Fungi
Microbiological count ADIVETER at days 7 and 14 Enterobacteriaceae Fungi Enterobacteriaceae Hongos Before pelleting Before pelleting T0 150000 67000 T0 4200 4700 Hydr sol Re-Hydra Pro day 7 day 14 Sol. Hidr. Re-Hydra Pro day 7 day 14 T0 20000 60000 T0 5000 35000 T0 20 30 T0 100 100 T0 10 10 T0 100 100 T0 30000 53000 T0 7000 20000 average 12507,5 28260 average 3050 13800 T1 0,25 10 10 T1 0,25 100 100 T1 0,25 2000 35000 T1 0,25 4200 8000 T1 0,25 10 10 T1 0,25 100 100 T1 0,25 10 10 T1 0,25 100 100 average 507,5 8757,5 average 1125 2075 T2 0,5 10 10 T2 0,5 100 100 T2 0,5 10 10 T2 0,5 100 100 T2 0,5 40 20000 T2 0,5 100 8000 T2 0,5 10 10 T2 0,5 100 100 average 17,5 5007,5 average 100 2075 T3 0,25 10 40 10 T3 0,25 10 300 100 T3 0,25 10 10 20 T3 0,25 10 100 400 T3 0,25 10 20 10 T3 0,25 10 200 100 T3 0,25 10 10 10 T3 0,25 10 100 100 average 20 12,5 average 175 175 T4 0,5 10 10 10 T4 0,5 10 100 100 T4 0,5 10 80 10 T4 0,5 10 100 100 T4 0,5 10 10 10 T4 0,5 10 100 100 T4 0,5 10 10 10 T4 0,5 10 100 100 average 27,5 10 average 100 100

56 Microbiological advantages after Re-Hydra Pro®
thousands of fungal cfu/ g -1 1 5 10 15 20 25 30 40 60 80 100 120 140 Basal Diet BD + 2% Water BD + 2% Water + tensioactive BD + 2% H20 Re-Hydra Pro® Storage days after pelleting The addition of total moisture (2%) reached levels of 12.4%, 12.8% and 13.7% for BD + 2% water, BD + 2% water + tensioactive and BD + 2% Water plus Re-Hydra Pro® (2%), respectively, after pelleting Storage conditions favoured a fast fungal growth (29ºC, RH: 85%) as accelerated tests for microbiological stability. The lower fungal growth after the application of Re-Hydra Pro® may be explained because of the control of the available water as well as because of the antifungal treatment applied through Re-Hydra Pro®. The fungal growth in BD + 2% water rose to the highest peaks because of the lack of proper feed protection with any kind of microbicidal treatment. The use of a tensioactive could not efficiently prevent the fungal growth.