J. Duranteau Hôpital de Bicêtre - Université Paris-Sud XI Réponse cellulaire à lhypoxie
Espèces radicalaires de loxygène Dysfonction dorgane Dysfonction endothéliale Réponse cellulaire à l hypoxie Hypoxie Métabolisme oxydatif Mitochondrie
Duranteau et al. J. Biol. Chem. 273: Duranteau et al. J. Biol. Chem. 273: Hepatocytes Cardiomyocytes
Duranteau et al. J. Biol. Chem. 273: Duranteau et al. J. Biol. Chem. 273:
Duranteau et al. J. Biol. Chem. 273: Duranteau et al. J. Biol. Chem. 273:
ADP + Pi ATP
ADP + Pi ATP NADH / FADH2 Flux délectrons ADP adenine nucleotide translocase Fuite protons
ADP + Pi ATP O 2.-
Guzy RD et al. Cell Metabolism 2005 Hep3B Human Hepatoma Cells
Prolyl hydroxylases Wheaton W and Chandel ND Am J Physiol Cell Physiol, Wheaton W and Chandel ND Am J Physiol Cell Physiol, 2010.
HIF-1-mediated expression of pyruvate dehydrogenase kinase: A metabolic switch required for cellular adaptation to hypoxia Pyruvate Deshydrogènase (PDH) Pyruvate Deshydrogènase kinase 1 Lactate deshydrogènase A Kim JW et al. Cell metabolism 2006
Wheaton W and Chandel ND Am J Physiol Cell Physiol, Wheaton W and Chandel ND Am J Physiol Cell Physiol, 2010.
Chen Z et al. Oncogene (2010) 29, 4362–4368
Comellas AP et al. Circ Res. 2006;98:
Gusarova AP et al. Molecular and Cellular Biology ATP demand
Réponse cellulaire à l hypoxie Hypoxie Diminution du métabolisme oxydatif Retarde la dysoxie cellulaire Diminue la production dERO Dysfonction dorgane
Aragones A et al. Nature Genetics 40, (2008) HIF prolyl hydroxylase 1-deficient (Egln2-/-) mice
Simerabet M et al.Brain research 2008
Piot C et al. NEJM 359; Effect of Cyclosporine on Reperfusion Injury in Acute Myocardial Infarction 58 patients with acute ST-elevation myocardial infarction intravenous bolus of 2.5 mg/kg of cyclosporine
Piot C et al. NEJM 359; Effect of Cyclosporine on Reperfusion Injury in Acute Myocardial Infarction 58 patients with acute ST-elevation myocardial infarction intravenous bolus of 2.5 mg/kg of cyclosporine
Espèces radicalaires de loxygène Dysfonction dorgane Dysfonction endothéliale Réponse cellulaire à l hypoxie Hypoxie Métabolisme oxydatif Mitochondrie Inflammation infection Inflammation infection
Tumor Necrosis Factor Inhibits Oxidative Phosphorylation through Tyrosine Phosphorylation at Subunit I of Cytochrome c Oxidase Bovine and murine hepatocyte homogenates CcO activity Cytochrome C (μM) 60% reduction in CcO activity 60% reduction in CcO activity Samavati L. et al. J. Biol. Chem. 283, pp –21144, 2008 Samavati L. et al. J. Biol. Chem. 283, pp –21144, 2008
Tumor Necrosis Factor Inhibits Oxidative Phosphorylation through Tyrosine Phosphorylation at Subunit I of Cytochrome c Oxidase ATP (%) TNFα Liver tissue H2.35 cells - + T T T T Samavati L. et al. J. Biol. Chem. 283, pp –21144, 2008 Samavati L. et al. J. Biol. Chem. 283, pp –21144, 2008
Espèces radicalaires de loxygène Dysfonction dorgane Dysfonction endothéliale Réponse cellulaire à l hypoxie Hypoxie Métabolisme oxydatif Mitochondrie