La présentation est en train de télécharger. S'il vous plaît, attendez

La présentation est en train de télécharger. S'il vous plaît, attendez

UMR CNRS 6022 Génie Enzymatique et Cellulaire (Directeur : A. Friboulet) Thème 1 : Biocatalyse et Alternatives Métaboliques (Prof. D. Thomas) Thème 2 :

Présentations similaires


Présentation au sujet: "UMR CNRS 6022 Génie Enzymatique et Cellulaire (Directeur : A. Friboulet) Thème 1 : Biocatalyse et Alternatives Métaboliques (Prof. D. Thomas) Thème 2 :"— Transcription de la présentation:

1 UMR CNRS 6022 Génie Enzymatique et Cellulaire (Directeur : A. Friboulet) Thème 1 : Biocatalyse et Alternatives Métaboliques (Prof. D. Thomas) Thème 2 : Biocatalyse supramoléculaire et anisotrope Métabolisme lipidique végétal (Dr. B. Thomasset, Dr. Y. Perrin) Génèse et caractérisation de la diversité biocatalytique (Dr. A. Friboulet, Dr. B. Bihan- Avalle, Dr. S. Padiolleau, Dr C. Bedel) Polymères biomimétiques, impression moléculaire et nanostructuration (Pr. K. Haupt, Dr A. Cordin) RMN des solides et systèmes biomimétiques (Pr. C. Sarazin) Modèles biomimétiques membranaires (Pr. S. Pulvin, Dr. S. Morandat, Dr. V. Ducel, Dr. C. Rossi)

2 Affinity separation BiosensorsBiochipsImmunoanalysis Directed synthesis and catalysis Antibodies Enzymes Hormon receptors Highly specific molecular recognition but Unstable Expensive Sometimes difficult to obtain SYNTHETIC RECEPTORS ? Drug targeting

3 Antigen MonomersComplexImprinted polymer MOLECULARLY IMPRINTED POLYMERS - SYNTHETIC ANTIBODIES

4 CHARACTERISTICS Tailor-made materials Physical Stability Chemical Stability Storage Endurance Imprint Memory Binding Strength Capacity Easy integration in industrial fabrication processes Resistant against mechanical stress, high pressures and temperatures up to 120ºC Resistant against acids, bases, various organic solvents, metal ions Years at RT without loss of performance Repeated use without loss of memory K D in nM - µM range 100 µg/g … 10 mg/g

5 APPLICATIONS Antibody / receptor binding site mimics Immunoassays Drug development / screening Sensors Chemical and Biosensors Multisensors and Biochips Tailor-made separation materials Facilitated synthesis / Catalysis (enzyme mimics) Slow release matrices Removal of unwanted molecules Biomedical, agriculture, food, textile,environment, security…

6 OUR ACTIVITIES Material engineering:Morphology (Films, nanoparticles…) Water-compatibility Recognition properties Surface engineering:Nanostructures Porosity Wetting properties Optical properties Separation:Selective extraction Chromatography Sensitive and selective Biomimetic assays detection:Acoustic and optical sensors Biochips MEMS BIOMIMETICS

7 ELISA-TYPE IMMUNOASSAY MIP nanospheres Conjugate 2,4-D Advantages No laboratory animals ! Fast and easy Sensitive High throughput Robotisable Chemiluminescence (H 2 O 2, luminol) N N H Tobacco peroxidase Detection: 2,4-D O C Cl O Antigen-enzyme conjugate H

8 Competitor, g/ml B/B 0, % Competitor 1: 2,4-Dichlorophenoxyacetic acid (2,4-D) 2: 4-Chlorophenoxyacetic acid 3: Phenoxyacetic acid 4: 2,4-D-methylester X-reactivity 100 % 10 % 2 % <1 % OCOOH Cl 2,4-D (Template) 1234 Competitor concentration 96-well plate 384-well plate

9 BIOPUCES Deposition of monomer solution with microcantilever array Imprinted polymer (model system) Trimethylolpropane trimethacrylate 4-Vinylpyridine Porogen : DMSO/Diglyme Antigen : Fluorescein Photopolymerisation (DPAP, 360 nm) Vandevelde et al. (2007) Langmuir 23,

10 NANOSTRUCTURED SURFACES BY NANOMOULDING

11

12

13 CHANGE OF SURFACE PROPERTIES Flat surface Nanostructured surface 0 min1 min3 min Poly(divinylbenzene-co-methacrylic acid) MIP Environmental scanning electron microscopy Condensation of water Water droplet on lotus leaf

14 SAS since 19 July 2004, Rouen, Normandy, France Founders : Kaynoush Naraghi Sami Bayoudh Karsten Haupt Developing innovative polymers : Molecularly imprinted polymers, responsive hydrogels, associative polymers Life and pharmaceutical sciences, food industry, agroindustry In 2004, winner of the Contest "Innovative and Technological Companies" of the French Ministry of Research&Technology Currently 10 people 3rd round of fund raising beginning 2008 VALORISATION


Télécharger ppt "UMR CNRS 6022 Génie Enzymatique et Cellulaire (Directeur : A. Friboulet) Thème 1 : Biocatalyse et Alternatives Métaboliques (Prof. D. Thomas) Thème 2 :"

Présentations similaires


Annonces Google